Protein Expression Lab



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Figure 1.1- Plate with antiboitic mixed in the agar showing no growth of E. coli BL21 colonies because the bacteria was not tranformed with DNA of a resistant strain.


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Figure 1.2- Plate with antiboitic mixed in the agar showing the growth of E. coli BL21 colonies after it has been tranformed with pGEM-gbr22.



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Figure 2- After overnight incubation, the mixture of LB, ampicillin, and resistant E. coli has become purple.


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Figure 4- Wet pellet of protein after the tube was centrifuged for 10 minutes at 5,000 rpm and extra liquid removed.